Coding

Part:BBa_K199195:Design

Designed by: Mary Gearing   Group: iGEM09_MoWestern_Davidson   (2010-12-12)


Cre with 8 bp restriction sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 377
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 63
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

5 bp spacer maintains reading frame


Source

Cre (J61047) + restriction sites and 5 bp spacer

References